Article | . Vol. 8, Issue. 1
Molecular Identification of Korean Polygonaceae: Weed Species



National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea1




.. 13:19


PDF XML




DNA barcode is a powerful tool for species identification. It is especially useful when identification is difficult due to lack of taxonomic knowledge. Plant DNA barcode employs two markers, rbcL + matK. There were 619 taxa, 81 families in agricultural fields in Korea and Polygonaceae is the fifth dominant among them and usually seven to eight species are commonly found. In this study, we tried to catalog 30 individuals of 7 species in Polygonaceae occurring in Korean agricultural fields using partial sequences of rbcL and matK. Both the barcode markers revealed a good PCR and sequencing success with 100%. Barcoding gap and resolving power were 100% as well. Interspecific variations were 3.23% and 7.87% in rbcL and matK, respectively. No intraspecific variations were found in Persicaria vulgaris and Rumex japonicus in both markers. Both standard markers were found to be useful for identification of Polygonaceae weeds in Korea. The barcode data were deposited in Genbank for public use.



1.  Brandbyge, J. 1993. Polygonaceae. In The Families and Genera of Vascular Plants. Vol. 2. Kubitzki, K. (ed.). pp. 531-544. Springer, Berlin, Heidelberg, Germany.  

2.  CBOL Plant Working Group. 2009. A DNA barcode for land plants. P. Natl. Acad. Sci. USA. 106:12794-12797.  

3.  Chen, S., Yao, H., Han, J., Liu, C., Song, J., et al. 2010. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS ONE 5 (1):e8613(8611-8618).  

4.  Dong, W., Gheng, T., Li, C., Xu, C., Long, P., et al. 2014. Discriminating plants using the DNA barcode Lb: An appraisal based on a large data set. Mol. Ecol. Res. 14:336-343.  

5.  Guo, X., Simmons, M.P., But, P.P.H., Shaw, P.C. and Wang, R.J. 2011. Application of DNA barcodes in L. (Spermacoceae, Rubiaceae). J. Syst. Evol. 49(3)203-212.  

6.  Hebert, P.D.N., Cywinska, A., Ball, S.L. and Waard, J.R.d. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B:Biological Sciences 270:313-321.  

7.  Heckenhauer, J., Barfuss, M.H.J. and Samuel, R. 2016. Universal multiplexable mat K primers for DNA barcoding of angiosperms. Applications in Plant Sciences. 4(6):1500137  

8.  Heckenhauer, J., Barfuss, M.H.J. and Samuel, R. 2016. Universal multiplexable mat K primers for DNA barcoding of angiosperms. Applications in Plant Sciences. 4(6):1500137.   

9.  Kim, C.S., Chung, Y.J., Oh, Y.J., Lee, I.Y., Cho, J.L., et al. 2008. A taxonomic study in early growing stage on the genus Polygonum (Polygonaceae) weeds of Korea. Kor. J. Weed Sci. 12:374-382. (In Korean)  

10. Kress, W.J. and Erickson, D.L. 2007. A two-locus global DNA barcode for land plants: The coding L gene complements the non-coding trnH-psbA spacer region. PLoS ONE 2(6):e508.   

11.  Lee, I.Y., Oh, Y.J., Park, J.S., Hong, S.H., Choi, J.K., et al. 2017. Occurrence characteristics of weed flora in arable fields of Korea. Weed Turf. Sci. 6(2):86-108. (In Korean)  

12.  Lee, J., Kim, C.S. and Lee, I.Y. 2015a. Molecular identification of Pooideae, Poaceae in Korea. Weed Turf. Sci. 1. (1):18-25. (In Korean)  

13.  FLee, J., Kim, C.S., Lee, I.Y., Oh, H.J., Kim, J.H., et al. 2015b. Identification of Korean Poaceae weeds based on DNA sequences. Weed Turf. Sci. 4(1):26-34.  

14. Park, C.W. and Hong, S.P. 2007. Polygonaceae. In the genera of vascular plants of Korea. Flora of Editorial  

15.  Park, J.E., Lee, I.Y., Moon, B.C., Park, T.S., Lim, S.T., et al. 2001. The occurrence characteristics of weed flora in rice paddy field of Korea. Kor. J. Weed Sci. 21(4):327-334. (In Korean)  

16.  Park, J.E., Lee, I.Y., Park, T.S., Lim, S.T., Moon, B.C., et al. 2003. Occurrence characteristics of weed flora in upland field of Korea. Kor. J. Weed Sci. 23(3):277-284. (In Korean)   

17.  Reveal, J.L. 1989. The Eriogonoid flora of California (Polygonaceae: Eriogonoideae). Phytologia 66:295-414.  

18.  Saitou, N. and Nei, M. 1987. The neighbor-joining method: A new method for reconstructing evolutionary trees. Mol. Bio. Evol. 4:406-425.  

19. Song, J.S. 2001. A systematic study on the genus Rumex (Polygonaceae) in Korea. Masters Thesis, Seoul  

20.  Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725-2729.  

21.  Theodoridis, S., Stefanaki, A., Tezcan, M., Aki, C., Kokkini, S., et al. 2012. DNA barcoding in native plants of the Labiatae (Lamiaceae) family from Chios Island (Greece) and the adjacent Çeşme-Karaburun Peninsula (Turkey). Mol. Ecol. Res. 12:620-633.  

22.  Thorne, R.F. 2000. The classification and geography of the flowering plants: Dicotyledons of the class Angiospermae. Bot. Rev. 66:441-647.  



online submission
Sub Menu
Sub Banner
Sub Banner
Sub Menu
Sub Banner
Sub Banner
Sub Banner
Sub Banner
Sub Menu
Sub Menu