Article | . Vol. 8, Issue. 1
Investigation of Resistance to Penoxsulam and Cyhalofop-butyl in Echinochloa oryzicola Collected in Gyeongsangnam-do Province of Korea

Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 52828, Korea1
Crop Protection Division, National Institute of Agricultural Sciences, RDA, Wanju 55365, Korea 2
충남대학교 농업생명과학대학 식물자원학과3
Department of Plant Medicine, IALS, Gyeongsang National University, Jinju 52828, Korea4

.. 67:74


In this study, Resistance of Echinochloa oryzicola biotypes collected in the rice fields of Gyeongnam province to both Cyhalofop-butyl and Penoxsulam were investigated by growth pouch assay. The collected biotypes showed a wide range of different sensitivities to both herbicides. For the standard known susceptible biotype, we used Sunchon biotype susceptible to Penoxsulam and Cyhalofop-butyl and determined as a Resistance if the GR50 value of a biotype is more than three times of the GR50 value of Sunchon biotype. Based on the GR50 values of 82 biotypes, 62.2% of biotypes were resistant to Penoxsulam, while 46.8% of biotypes were resistant to Cyhalofop-butyl. The Resistance/susceptible (R/S) GR50 ratio of the highest resistant biotype to Penoxsulam compared to the Sunchon biotype was 252.76 (Hamyang, HY-11 biotype), while that of the highest resistant biotype to Cyhalofop-butyl was 23.39 (Jinju, JJ-18 biotype).

1.  Aung, B.B., Won, O.J., Sin, H.T., Lee, J.J. and Park, K.W. 2017. Mechanisms of herbicide in weeds.Kor. J. Agric. Sci. 44(1):1-15.  

2.  Beckie, H.J., Friesen, L.F., Nawolsky, K.M. and Morrison, I.N. 1990. A rapid bioassay to detect trifluralinresistant green foxtail (Setaria viridis). Weed Technol. 4:505-508.   

3.  Heap, I. 2019a. The international survey of herbicide resistant weeds. (Accessed Mar. 05, 2019)   

4.  Heap, I. 2019b. The international survey of herbicide resistant weeds. ResistantWeed/Species.aspx (Accessed Mar. 06, 2019).  

5.  Hensley, J.R. 1981. A method for identification of triazine resistant and susceptible biotypes of several weeds. Weed Sci. 29:70-73.  

6.  Holm, L.G., Plucknett, D.L., Pancho, J.V. and Herberger, J.P. 1977. The world’s worst weeds: Distribution and biology. pp. 107-110. University Press of Hawaii, Hawaii, USA.  

7.  Holt, J.S., Holtum, J.A.M. and Powles, S.B. 1993. Mechanisms and agronomic aspects of herbicide , Annu. Rev. Plant Physiol. Plant Mol. Biol. 44:203-229.  

8.  HRAC (Herbicide Action Committee). 2019. Detecting herbicide . http:// (Accessed Mar. 06, 2019).  

9.  Im, I.B. 2009. Control and emergence of herbicides resistant in paddy field of Korea. Kor. J. Weed Sci. 29(Supp 2):103-104. (In Korean)   

10.  Im, S.H., Park, M.W., Yook, M.J. and Kim, D.S. 2009. to ACCase inhibitor in Echinochloa crus-galli var. crus-galli collected in Seosan, Korea. Kor. J. Weed Sci. 29(2):178-184.  

11.  Kaundun, S.S., Hutchings, S.J., Dale, R.P., Bailly, G.C. and Glanfield, P. 2011. Syngenta ‘RISQ’ test: A novel in-season method for detecting to post-emergence ACCase and ALS inhibitor herbicides in grass weeds. Weed Res. 51:284-293.  

12.  Kaundun, S.S. and Windass, J.D. 2006. Derived cleaved amplified polymorphic sequence, a simple method to detect a key point mutation conferring acetyl-CoA carboxylase inhibitor herbicide in grass weeds. Weed Res. 46:34-39.  

13.  Kim, D.S., Caseley, J.C., Brain, P., Riches, C.R. and Valverde, B.E. 2000. Rapid detection of propanil and fenoxaprop in Echinochloa colona. Weed Sci. 48:695-700.  

14.  Lee, I.Y., Kim, C.S., Lee, J.R., Park, T.S., Moon, B.C., et al. 2017. Changes in weed vegetation in paddy fields over the last 50 years in Korea. Weed Turf. Sci. 5(1):1-4. (In Korean)  

15.  Lee, Y.H, Shim, S.Y., Kim, J.W, Lee, J.R, Park, K.W., et al. 2018. Occurrence and distribution of ALS inhibiting herbicide-resistant weeds in the paddy field of Gyeongnam province. Weed Turf. Sci. 7:209-218. (In Korean)  

16.  Marshall, R. and Moss, S.R. 2008. Characterization and molecular basis of ALS inhibitor in the grass weed Alopecurus myosuroides. Weed Res. 48:439-447  

17.  Moss, S.R. 1995. Techniques for determining herbicide . pp. 547-556. In: Proc. Brighton Crop Prot. Conf. Weeds. British Crop Protection Council, Farnham, UK.  

18.  Norsworthy, J.K., Talbert, R.E. and Hoagland, R.E. 1998. Chlorophyll fluorescence for rapid detection of propanil resistant barnyardgrass (Echinochloa crus-galli). Weed Sci. 46:163-169.  

19.  Park, T.S., Ku, B.I., Kang, S.K., Choi, M.K., Park, H.K., et al. 2010. Response of the resistant biotype of Echinochloa orizoides to ACCase and ALS inhibitors, and effect of alternative herbicides. Kor. J. Weed Sci. 30(3):291-299. (In Korean)  

20.  Prather, T.S., Ditomaso, J.M. and Holt, J.S. 2000. Herbicidie : Definition and management strategies. Publication 8012, California, USA.  

21.  Reade, J.P. and Cobb, A.H. 2002. New, quick tests for herbicide in blackgrass (Alopecurus myosurodes Huds.) based on increased glutathione S-transferase activity and abundance. Pest Manag. Sci. 58:26-32.  

22.  Richter, J. and Powles, S.B. 1993. Pollen expression of herbicide target site genes in annual ryegrass (Lolium rigidum). Plant Physiol. 102: 1037-1041.  

23.  Zhang, C.J., Im, S.H., Kim, J.W., Song, J.S., Yook, M.J., et al. 2015. Quantifying herbicide dose-response and in Echinochloa spp. by measuring root length in es. Can. J. Plant Sci. 95: 1181-1192.  

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